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Ivan Scheblykin. Portrait.

Ivan Scheblykin

Professor

Ivan Scheblykin. Portrait.

Fast-tracking of single emitters in large volumes with nanometer precision

Author

  • BORIS LOUIS
  • RAFAEL CAMACHO
  • ROGER BRESOLÍ-OBACH
  • SERGEY ABAKUMOV
  • JOHANNES VANDAELE
  • TESTSUHIRO KUDO
  • HIROSHI MASUHARA
  • IVAN G. SCHEBLYKIN
  • JOHAN HOFKENS
  • SUSANA ROCHA

Summary, in English

Multifocal plane microscopy allows for capturing images at different focal planes simultaneously. Using a proprietary prism which splits the emitted light into paths of different lengths, images at 8 different focal depths were obtained, covering a volume of 50x50x4 μm3. The position of single emitters was retrieved using a phasor-based approach across the different imaging planes, with better than 10 nm precision in the axial direction. We validated the accuracy of this approach by tracking fluorescent beads in 3D to calculate water viscosity. The fast acquisition rate (>100 fps) also enabled us to follow the capturing of 0.2 μm fluorescent beads into an optical trap.

Department/s

  • Chemical Physics
  • NanoLund: Centre for Nanoscience

Publishing year

2020

Language

English

Pages

28656-28671

Publication/Series

Optics Express

Volume

28

Issue

19

Document type

Journal article

Publisher

Optical Society of America

Topic

  • Atom and Molecular Physics and Optics

Status

Published

ISBN/ISSN/Other

  • ISSN: 1094-4087